Development of an IMS-PCR assay for the detection of Mycobacterium avium ssp. paratuberculosis in water
نویسندگان
چکیده
منابع مشابه
Mycobacterium avium ssp. paratuberculosis vs Crohn’s Disease
Crohn's disease (CD) is a chronic inflammatory bowel disease, with the potential to affect any segment of the gastrointestinal tract. Despite the great advances in recent decades, which provided a better understanding of the pathogenesis of the disease, it is yet to be completely elucidated. Presence of genetic factors, luminal factors such as microflora and factors related to the intestinal ba...
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Mycobacterium avium subspecies paratuberculosis (MAP) is the cause of John’s disease also called paratuberculosis. This is economically one of the important infectious diseases in cattle and ruminant husbandry. This disease is manifested as granulomatosis entrocolitis, lymphadenitis and inflammation local lymphatic vessels. The typical sign of this disease is progressive loss of weight. Cons...
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The efficacy of bacterial cultures and IS900-specific polymerase chain reaction (PCR) was compared for the detection of Mycobacterium avium subsp. paratuberculosis (MAP) from the mesenteric lymph nodes of goats. Samples were collected from 75 goats slaughtered in Ilam, in southwest of Iran. Tissue homogenates were inoculated onto four media. The genomic DNA was extracted directly from mesenteri...
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15 صفحه اولDevelopment of a Recombinase Polymerase Amplification Assay for Rapid Detection of the Mycobacterium avium subsp. paratuberculosis
BACKGROUND The detection of Mycobacterium avium subsp. paratuberculosis (MAP) infections in ruminants is crucial to control spread among animals and to humans. Cultivation of MAP is seen as the gold standard for detection, although it is very time consuming and labour intensive. In addition, several PCR assays have been developed to detect MAP in around 90 minutes, but these assays required hig...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2005
ISSN: 0266-8254,1365-2673
DOI: 10.1111/j.1472-765x.2005.01662.x